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生物来源
mouse
质量水平
抗体形式
purified antibody
克隆
1C8, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型
IgG2b
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.
特异性
Reacts with human Erythropoietin (Epo).
免疫原
Recombinant human Epo.
应用
Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Metabolic Hormones & Receptors
Metabolic Hormones & Receptors
质量
Evaluated by Western Blot on Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
目标描述
34 kDa (glycosylated)
外形
Protein A purified
Format: Purified
Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
储存及稳定性
Stable for 1 year at 2-8ºC from date of receipt.
分析说明
Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Thomas H Sanderson et al.
PloS one, 8(11), e78627-e78627 (2013-11-14)
Recent advancements in isolation techniques for cytochrome c (Cytc) have allowed us to discover post-translational modifications of this protein. We previously identified two distinct tyrosine phosphorylated residues on Cytc in mammalian liver and heart that alter its electron transfer kinetics
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