Anti-Hepatitis B Virus Surface Antigen Antibody, clone H21F8-1

Large envelope protein (UniProt: A7XR30; also known as L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen) is encoded by the S (also known as Large S, P, preS1, preS2) gene (Gene ID: 944569) in Hepatitis B virus (HBV). HBV is a small enveloped virus of the Hepadnaviridae family with a partially double-stranded DNA genome approximately 3.2 kb. Hepatitis B surface antigen is a distinctive serological marker of acute or chronic hepatitis B infection and is the first antigen to appear following infection with hepatitis B virus. HBV contains three viral envelope/surface proteins - large surface antigen, middle surface antigen, and small surface antigen. They are expressed from a single open reading frame. The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and is not N-glycosylated. It is shown to exist in two topological conformations, one which is termed ′external′ or Le-HBsAg and the other ′internal′ or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. (Ref.: Wu, CC., et al. (2018). World J. Gastroenterol. 24(31);: 3488 3499).

Clone H21F8-1 is a mouse monoclonal antibody that detects large envelope protein of Hepatitis B virus.

Hepatitis B virus surface antigen (HBsAg).

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Hepatitis B Virus Surface Antigen in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Anti-Hepatitis B Virus Surface Antigen, clone H21F8-1, Cat. No. MAB10234-I, is a mouse monoclonal antibody that detects Large envelope protein of Hepatitis B virus and is tested for use in Immunohistochemistry (Paraffin).

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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