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MAB030

Sigma-Aldrich

抗DNA抗体,双链,克隆BV16-13

culture supernatant, clone BV16-13, Chemicon®

别名:

Double-stranded DNA antibody

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

culture supernatant

抗体产品类型

primary antibodies

克隆

BV16-13, monoclonal

种属反应性(根据同源性预测)

all

制造商/商品名称

Chemicon®

技术

ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable

同位素/亚型

IgG2aκ

运输

wet ice

靶向翻译后修饰

unmodified

特异性

来自人的双链DNA(dsDNyA)(B型)。显示对dsDNA的反应性为95%,对ssDNA的反应性为28%。

结合试验:

将上清液与32P末端标记的热变性(ss)DNA或双链(ds)DNA限制性片段(20 pmoles核苷酸;16,000 cpm)培养,然后用过量的兔抗小鼠沉淀抗DNA/DNA复合物并定量通过 Chernkov辐射结合的[32P]DNA。



抗原结合

特征: 相对结合:



天然DNA 合成均聚物

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



核酸与固相复合mBSA相复合与抗DNA抗体一起培养。测量在最高测试抗体量(10 mg纯化抗体或1:2培养上清液稀释液)下的放射性碘化蛋白A结合,并相对于针对优选抗原确定的结合(给定值为1.0)进行标准化。



a)天然小牛胸腺DNA b)热变性小牛胸腺DNA

免疫原

表位:双链,28%与单链反应

应用

EIA/RIA

免疫细胞化学

最佳工作稀释度必须由最终用户确定。
用抗DNA抗体双链克隆BV16-13(小鼠单克隆抗体)检测DNA,该抗体已证明在ELISA,RIA,ICC中有效。
研究子类别
细胞周期,DNA复制 & 修复

细胞凋亡-其他
研究类别
表观遗传学&核功能

细胞凋亡&癌症

外形

来自灌注系统的未纯化组织培养物上清液,在装入小瓶之前通过0.2μ微米膜过滤。产品包含20%FBS和环丙沙星,最终浓度为10μg/ml。

储存及稳定性

以未稀释的等分试样保存于2-8°C下6个月。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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