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Merck
CN
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文件

安全信息

LI0685BC

Millipore

TRANSIA® PLATE Listeria spp. 10X

BioControl, ELISA test for detection of Listeria in food and environmental samples

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别名:
Listeria detection kit
UNSPSC代码:
41116129
NACRES:
NA.85

Agency

AFNOR

产品线

TRANSIA®

保质期

3‑24 mo.

包装

pkg of 960 tests

制造商/商品名称

BioControl Systems LI0685

技术

ELISA: suitable

输入

food(s)

应用

food and beverages
pathogen testing

储存温度

2-8°C

适用性

Listeria spp.

相关类别

一般描述

TRANSIA® PLATE Listeria spp. 10X is an ELISA test kit that incorporates proven antibody-antigen "sandwich" assay technology for the detection of Listeria species in both food and environmental samples. Highly specific antibodies coated to polystyrene microtiter plate wells capture and bind the target antigen, if present. A detection antibody linked to a conjugate enzyme is introduced to form an antibody/antigen/antibody sandwich. Finally, a substrate is added and converted by the conjugate enzyme to produce a color change indicating the presence of the target pathogen. The extensive AOAC and AFNOR validations and built-in positive and negative controls combine to provide users with the necessary confidence in the results. The efficient divisible 96-well format and available automation make it an ideal solution for high-volume testing.

应用

TRANSIA® PLATE Listeria is a kit for detection of the Listeria (L. monocytogenes, L. ivanovii, L. innocua, L. seeligeri, L. welshimeri, and L. grayi) in dairy products, meat products, seafood products, vegetables, and environmental samples. The assay reliably recovers and detects low levels of Listeria spp. in a variety of samples.

特点和优势

  • Detects all 6 species of Listeria providing a true indication of product and surface quality.
  • Positive and Negative controls supplied with the kit.

组分

  • 10 microplates with dividable microwell strips with 96 wells (12 microwell strips of 8 microwells).
  • Negative control: (bacterial antigens not belonging to the Listeria genus), ready-to-use, 2 x 20 mL.
  • Positive control: Listeria monocytogenes antigens, ready-to-use, 2 x 10mL.
  • Conjugate (contains peroxidase conjugated anti-Listeria antibodies), ready-to-use, 2 x 57 mL.
  • Wash buffer, 20 X concentration, 800 mL.
  • Substrate (urea H2O2), ready-to-use, 1 x 57 mL.
  • Chromogen (TMB), ready-to-use, 1 x 57 mL.
  • Stop solution (H2SO4), ready-to-use, 2 x 57 mL.

原理

TRANSIA® PLATE Listeria is an ELISA test (Enzyme Linked Immuno Sorbent Assay) based on a two step sandwich-type reaction. The solid support of the reaction is a microtitration plate with dividable microwellstrips. Specific antibodies of flagellar antigens are grafted on the microtitration plate wells.

测试方法

ELISA

法律信息

TRANSIA is a registered trademark of Merck KGaA, Darmstadt, Germany

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 1

靶器官

Eyes,Central nervous system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品
常规特殊物品
含少量动物源组分生物产品

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