产品名称
蛋白质G Plus /蛋白质A琼脂糖悬浮液, Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
form
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
technique(s)
protein purification: suitable
suitability
suitable for microbiology
shipped in
wet ice
storage temp.
2-8°C
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
免疫沉淀法（参见评论）
Disclaimer
毒性：标准处理（A）
General description
专为免疫沉淀应用而设计。该产品已使用 BSA 进行封闭，以减少非特异性结合，不能用于纯化。
专门配制用于免疫沉淀的蛋白 G PLUS/蛋白 A 琼脂糖混合物。
Other Notes
提供琼脂糖溶液以备使用。蛋白 G Plus/蛋白 A 琼脂糖免疫沉淀试剂被 BSA 封闭，不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应，建议每 µg 一抗加入 15 µl 溶液。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。
Physical form
PBS 中 33％ 的浆液。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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