推荐产品
形式
slurry (Liquid)
包含
≤0.1% sodium azide as preservative
制造商/商品名称
Calbiochem®
储存条件
do not freeze
技术
immunoprecipitation (IP): suitable
适用性
suitable for immunoprecipitation
运输
wet ice
储存温度
2-8°C
一般描述
专为免疫沉淀应用而设计。本品用BSA阻断，以减少非特异性结合，不能用于纯化；最适合牛，山羊，人和大鼠IgG和小鼠IgG1。
蛋白G PLUS琼脂糖混悬剂，专门配制用于免疫沉淀。
警告
毒性：标准处理（A）
其他说明
提供琼脂糖溶液以备使用。蛋白G Plus琼脂糖被BSA阻断，不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应，建议使用15 µl/µg一抗。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Leo K Iwai et al.
Journal of proteome research, 9(6), 3135-3145 (2010-05-05)
Type 1 diabetes, in human patients and NOD mice, results from an immune attack on insulin-producing beta-cells of the pancreas by autoreactive T lymphocytes. In NOD mice, genetically controlled perturbations in the signaling pathways downstream of the antigen-specific T cell
Xin Wang et al.
The Journal of biological chemistry, 294(43), 15733-15742 (2019-09-06)
REV7, also termed mitotic arrest-deficient 2-like 2 (MAD2L2 or MAD2B), acts as an interaction module in a broad array of cellular pathways, including translesion DNA synthesis, cell cycle control, and nonhomologous end joining. Numerous REV7 binding partners have been identified
Matthew M Halpert et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 8082-8101 (2020-04-17)
Mammalian immune responses are initiated by "danger" signals--immutable molecular structures known as PAMPs. When detected by fixed, germline encoded receptors, pathogen-associated molecular pattern (PAMPs) subsequently inform the polarization of downstream adaptive responses depending upon identity and localization of the PAMP.
Matthew M Halpert et al.
Stem cells and development, 25(10), 774-787 (2016-03-17)
The remarkable functional plasticity of professional antigen-presenting cells (APCs) allows the adaptive immune system to respond specifically to an incredibly diverse array of potential pathogenic insults; nonetheless, the specific molecular effectors and mechanisms that underpin this plasticity remain poorly characterized.
Boyang Zhao et al.
American journal of physiology. Cell physiology, 302(1), C27-C45 (2011-09-24)
Although extensive phosphoproteomic information is available for renal epithelial cells, previous emphasis has been on phosphorylation of serines and threonines with little focus on tyrosine phosphorylation. Here we have carried out large-scale identification of phosphotyrosine sites in pervanadate-treated native inner
相关内容
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
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