推荐产品
表单
slurry (Liquid)
包含
≤0.1% sodium azide as preservative
制造商/商品名称
Calbiochem®
储存条件
do not freeze
运输
wet ice
储存温度
2-8°C
一般描述
与琼脂糖结合的蛋白A。
专为免疫沉淀应用而设计。本品用BSA封闭，减少非特异性结合，不能用于纯化；对小鼠IgG2a和IgG2b及兔IgG效果最好。
警告
毒性：标准处理（A）
外形
在PBS中。
其他说明
琼脂糖溶液为即用型，可用于免疫沉淀。使用15µ l混悬剂/µg一抗。推荐将蛋白A-琼脂糖用于使用兔多克隆抗体或未修饰的小鼠IgG2a或IgG2b单克隆抗体的免疫沉淀测定。小鼠IgG1或IgG3和全部大鼠抗体与蛋白A的结合较弱。作为替代方案，使用蛋白G PLUS，蛋白G PLUS/蛋白A或兔第二步抗体将这些抗体与蛋白A桥接。预清除将使由非特异性沉淀产生额外的条带最小化。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与BSA或样品中存在的其他高浓度蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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