IF005
干扰素-γ蛋白，重组小鼠
The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.
别名:
IFN γ, IFN g, IFN gamma, INF γ, INF g, INF gamma
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About This Item
UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.77
一般描述
产品来源：小鼠，在大肠埃希菌中表达
干扰素-γ（IFN-γ）是由活化的NK细胞和CD4+TCRα/β+，CD8+TCRα/β+和TCRγ/δ+T细胞产生的调节蛋白。 IFN-γ的生物活性包括与一类高亲和力受体的特异性结合。 此外，IFN-γ可以升级淋巴样细胞功能的数量，包括巨噬细胞，NK细胞和中性粒细胞的抗微生物和抗肿瘤反应。重组小鼠IFN-γ是一种含134个氨基酸的蛋白，预测分子量为15.6 kDa。在大肠杆菌发酵过程中由于蛋白水解裂解出现C末端截短，并产生一种经质谱验证为15.1 kDa的蛋白。重组小鼠IFN-γ的SDS-PAGE 分析出现一条11-12 kDa的条带，在非还原和还原条件下，该条带在11-12 kDa左右迁移。
应用
对于大多数体外应用，IFN-γ在0.1至10.0 ng/mL的浓度范围内发挥其生物活性。
生化/生理作用
用小鼠NIH 3T3细胞攻击的VSV通过细胞病变效应抑制试验确定的ED50为≤0.1 ng/ml
外形
10 mM磷酸钠、pH值7.0+25 mM NaCl经无菌过滤、冻干。
储存及稳定性
尽管冻干的蛋白质在室温下可稳定数周，但最好在 -20°C 下保存。快速旋转后，用磷酸钠，pH值8.0复溶至1 mg/mL的浓度。 进一步稀释应配成RPMI+10%FCS或5%HAS在2-8ºC保存一周或在-20ºC保存备用。自收到之日起，复溶的IFN-γ应该以未稀释的等分试样在 -20°C 下保存长达 6 个月。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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