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HDIAB-34K-PMX5

Millipore

MILLIPLEX® Human Diabetes Panel, Premixed 5 Plex Assay

The Human Diabetes, Premixed 5 Plex Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 protein biomarkers in human serum, plasma and cell culture samples.

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000

种属反应性

human

制造商/商品名称

Milliplex®

assay range

accuracy: 86%
(GLP-1 (Active))

accuracy: 91%
(Insulin)

accuracy: 92%
(C-Peptide)

accuracy: 94%
(Leptin)

sensitivity: 15.8 pg/mL
(MinDC+2SD; Glucagon)

sensitivity: 16.4 pg/mL
(MinDC+2SD; C-Peptide)

sensitivity: 2.1 pg/mL
(MinDC+2SD; GLP-1 (Active))

sensitivity: 32.7 pg/mL
(MinDC+2SD; Insulin)

sensitivity: 65.9 pg/mL
(MinDC+2SD; Leptin)

standard curve range: 13.7-10,000 pg/mL
(Glucagon)

standard curve range: 137-100,000 pg/mL
(Insulin)

standard curve range: 137-100,000 pg/mL
(Leptin)

standard curve range: 2.7-2,000 pg/mL
(GLP-1 (active))

standard curve range: 27.4-20,000 pg/mL
(C-Peptide)

inter-assay cv: <15%
intra-assay cv: <10%
(C-Peptide)

inter-assay cv: <15%
intra-assay cv: <10%
(Glucagon)

inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)

inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)

inter-assay cv: <20%
intra-assay cv: <10%
(GLP-1 (Active))

技术

multiplexing: suitable

相容性

configured for Premixed

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

一般描述

Type I Diabetes is an early onset autoimmune disease that destroys insulin producing pancreatic β-cells. Type II Diabetes is later onset metabolic disorder caused by obesity, poor diet and a sedentary lifestyle. With insufficient levels of insulin, or resistance to insulin, elevated glucose can lead to heart disease, stroke, kidney failure and dementia.

MILLIPLEX® Human Diabetes Premixed Bead Panel is a 5-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma, or culture supernatant samples: C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin.

This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

特异性

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

应用

  • Analytes: C-Peptide, Glucagon-like Peptide-1 (GLP-1) (Active), Glucagon, Insulin, Leptin
  • Recommended Sample Type: Human serum, plasma, tissue/cell culture supernatants and lysates
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require a dilution with an appropriate control medium.
  • NOTE: Protease inhibitors must be added to blood upon collection, DPPIV inhibitor for GLP-1 and Aprotinin for Glucagon. See protocol for details.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Obesity, Diabetes, Diabetes - Type 1, Diabetes - Type 2

包装

Everything you need in a single kit.

储存及稳定性

Recommended storage for kit components is 2 - 8°C.

其他说明

Please contact Technical Service for linearity of dilution.

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Skull and crossbonesEnvironment

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

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Sergejs Berdnikovs et al.
The Journal of allergy and clinical immunology, 149(5), 1802-1806 (2021-11-07)
The roles of systemic and airway-specific epithelial energy metabolism in altering the developmental programming of airway epithelial cells (AECs) in early life are poorly understood. Our aim was to assess carbohydrate metabolism in developing AECs among children with and without

相关内容

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

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