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种属反应性
human
制造商/商品名称
Milliplex®
assay range
accuracy: 86%
(GLP-1 (Active))
accuracy: 91%
(Insulin)
accuracy: 92%
(C-Peptide)
accuracy: 94%
(Leptin)
sensitivity: 15.8 pg/mL
(MinDC+2SD; Glucagon)
sensitivity: 16.4 pg/mL
(MinDC+2SD; C-Peptide)
sensitivity: 2.1 pg/mL
(MinDC+2SD; GLP-1 (Active))
sensitivity: 32.7 pg/mL
(MinDC+2SD; Insulin)
sensitivity: 65.9 pg/mL
(MinDC+2SD; Leptin)
standard curve range: 13.7-10,000 pg/mL
(Glucagon)
standard curve range: 137-100,000 pg/mL
(Insulin)
standard curve range: 137-100,000 pg/mL
(Leptin)
standard curve range: 2.7-2,000 pg/mL
(GLP-1 (active))
standard curve range: 27.4-20,000 pg/mL
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
(Glucagon)
inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)
inter-assay cv: <20%
intra-assay cv: <10%
(GLP-1 (Active))
技术
multiplexing: suitable
相容性
configured for Premixed
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
MILLIPLEX® Human Diabetes Premixed Bead Panel is a 5-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma, or culture supernatant samples: C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin.
This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
特异性
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
应用
- Analytes: C-Peptide, Glucagon-like Peptide-1 (GLP-1) (Active), Glucagon, Insulin, Leptin
- Recommended Sample Type: Human serum, plasma, tissue/cell culture supernatants and lysates
- Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require a dilution with an appropriate control medium.
- NOTE: Protease inhibitors must be added to blood upon collection, DPPIV inhibitor for GLP-1 and Aprotinin for Glucagon. See protocol for details.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Obesity, Diabetes, Diabetes - Type 1, Diabetes - Type 2
包装
储存及稳定性
其他说明
法律信息
免责声明
警示用语:
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
相关内容
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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