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HCCB4MAG-58K

Millipore

MILLIPLEX® Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84

种属反应性

human

质量水平

制造商/商品名称

Milliplex®

assay range

accuracy: 80-110%
standard curve range: 0.1-100 ng/mL
(L1CAM)

standard curve range: 0.1-100 ng/mL
(Mesothelin)

standard curve range: 0.3-250 ng/mL
(Hepsin)

standard curve range: 1.4-1,000 ng/mL
(CD44)

standard curve range: 1.4-1,000 ng/mL
(Transglutaminase 2 (TGM2))

standard curve range: 13.7-10,000 pg/mL
(EpCAM)

standard curve range: 13.7-10,000 pg/mL
(Midkine)

standard curve range: 2.7-2,000 ng/mL
(ALDH1A1)

standard curve range: 20.6-15,000 pg/mL
(Carbonic Anhydrase IX (CA9))

standard curve range: 27.4-20,000 pg/mL
(Kallekrein-6)

inter-assay cv: <10%
intra-assay cv: <20%

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

一般描述

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX® Human Circulating Cancer Biomarker Panel 4 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), and Transglutaminase 2 (TGM2). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer
MILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.

应用

  • Analytes: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), Transglutaminase 2 (TGM2)
  • Recommended Sample Type: Human serum, plasma or tissue/cell/tumor culture supernatant
  • Recommended Sample Dilution: 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • NOTE: min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL
  • Research Category: Cancer
  • Research Subcategory: Immune Response, Alzheimer′s Disease, Inflammation, Oncology, Tumor Markers

特点和优势

Design your multiplex kit by choosing available analytes within this panel.

其他说明

Please contact Technical Service for linearity of dilution.
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

靶器官

Respiratory Tract

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Imad Tarhoni et al.
Journal of immunoassay & immunochemistry, 39(1), 84-98 (2018-01-09)
Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. Antigen "capture" antibodies for

相关内容

Cancer biomarker detection through MILLIPLEX® multiplex cancer assays can accelerate studies of tumorigenic processes helping advance cancer research.

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