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HATF00010

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

别名:

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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关于此项目

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red
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产品名称

Immobilon®-NC转移膜, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

Quality Level

400

material

mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter

feature

hydrophilic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

33 cm × 3 m

pore size

0.45 μm pore size

capacity

117 μg/cm2 adsorption capacity (insulin), 160 μg/cm2 adsorption capacity (BSA), 259 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

Immobilon-NC (HATF)膜由混合纤维素酯基质组成。它缺乏表面活性剂，在细胞生长过程中可能会干扰细胞壁的完整性。这种膜推荐用于菌落和菌斑提升。

Application

Immobilon-NC转移膜已用于蛋白质印迹分析。

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

Hazard Classifications

Flam. Sol. 1

存储类别

4.1B - Flammable solid hazardous materials

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实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

Bing Wang et al.
Oncology letters, 13(5), 3494-3500 (2017-05-23)
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R)
Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
Peiying Tian et al.
Cell biology international, 44(8), 1681-1690 (2020-04-14)
Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The
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全球贸易项目编号

货号GTIN
HATF0001004053252672811