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Merck
CN
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文件

HATF00010

Millipore

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

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别名:
Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane
UNSPSC代码:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-NC转移膜, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

物料

mixed cellulose esters (MCE) membrane
nitrocellulose membrane
plain filter
white filter

质量水平

400

特点

hydrophilic

制造商/商品名称

Immobilon®

技术

western blot: suitable

过滤器长度 × 宽度

33 cm × 3 m

孔径

0.45 μm pore size

容量

117 μg/cm2 adsorption capacity (insulin)
 160 μg/cm2 adsorption capacity (BSA)
 259 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with India ink
for use with Ponceau-S red

检测方法

chemiluminescent
colorimetric
fluorometric
radioactive

运输

ambient

相关类别

一般描述

Immobilon-NC (HATF)膜由混合纤维素酯基质组成。它缺乏表面活性剂，在细胞生长过程中可能会干扰细胞壁的完整性。这种膜推荐用于菌落和菌斑提升。

应用

Immobilon-NC转移膜已用于蛋白质印迹分析。

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Flame
GHS02

警示用语：

Danger

危险声明

H228

危险分类

Flam. Sol. 1

WGK

WGK 3

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Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
Bing Wang et al.
Oncology letters, 13(5), 3494-3500 (2017-05-23)
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R)
Hong Wen et al.
Reproductive sciences (Thousand Oaks, Calif.), 27(6), 1357-1364 (2020-02-15)
Selective intrauterine growth restriction (sIUGR) is a disorder of monochorionic (MC) twin pregnancies. However, the underlying mechanism remains largely unknown. Trophoblast cells are the major component of the placenta. Dysfunction of trophoblast cells is associated with placental dysfunction. Our previous
Kuanxiang Sun et al.
BioMed research international, 2020, 7342817-7342817 (2020-07-01)
Phosphatidylserine flippase (P4-ATPase) transports PS from the outer to the inner leaflet of the lipid bilayer in the membrane to maintain PS asymmetry, which is important for biological activities of the cell. ATP11A is expressed in multiple tissues and plays
Yongping Zhou et al.
Cancer cell international, 20, 225-225 (2020-06-17)
Pancreatic cancer is a highly malignant gastrointestinal cancer that can widely metastasize during the early stage of disease, and it is associated with one of the worst prognoses among cancers. In this study, we aimed to investigate the function of
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实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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