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HADK2MAG-61K

Millipore

MILLIPLEX® Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of nine protein biomarkers in human serum and plasma samples.

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.47

质量水平

200

种属反应性

human

制造商/商品名称

Milliplex®

assay range

accuracy: 87%
(IL-8)
accuracy: 91%
(TNFα)
accuracy: 94%
(Insulin)
standard curve range: 0.64-10,000 pg/mL
(IL-8)
standard curve range: 0.64-10,000 pg/mL
(NGF)
standard curve range: 0.64-10,000 pg/mL
(TNF-α)
standard curve range: 0.96-15,000 pg/mL
(IL-6)
standard curve range: 1.3-20,000 pg/mL
(IL-1β)
standard curve range: 1.3-20,000 pg/mL
(MCP-1)
standard curve range: 38-600,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(HGF)
standard curve range: 9.6-150,000 pg/mL
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-1β)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

一般描述

Previously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

The MILLIPLEX® Human Adipokine Panel 2 is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: HGF, IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 samples in duplicate.

Panel Type: Metabolism

特异性

Cross Reactivty
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.

应用

  • Analytes: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-α
  • Recommended Sample Type: Human serum and plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

Download the HADK2MAG-61K xPonentProtocol Template Version 3.1

特点和优势

Design your multiplex kit by choosing available analytes within this panel.

包装

Everything you need in a single kit.
96-well plate

储存及稳定性

Recommended storage for kit components is 2 - 8°C.

其他说明

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual sensitives.

法律信息

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Skull and crossbonesEnvironment
GHS06,GHS09

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

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The role of neurotrophic factors has been highlighted as a cause of decline in the cognitive function of alcohol-dependent patients. It is known that nerve-growth factor (NGF), one of the neurotrophins, is related to the growth and differentiation of nerve
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