跳转至内容
Merck
CN
所有图片(3)

主要文件

查看所有文档

安全信息

HADCYMAG-61K

Millipore

MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay

The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.

登录查看公司和协议定价
所有图片(3)

About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84

质量水平

200

种属反应性

human

制造商/商品名称

Milliplex®

assay range

accuracy: 100%
(IL-6)
accuracy: 102%
(TNFα)
accuracy: 103%
(MCP-1)
accuracy: 83%
(PAI-1 (total))
accuracy: 99%
(HGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; IL-8)
sensitivity: 0.2 pg/mL
(MinDC+2SD; NGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; TNF?)
sensitivity: 0.6 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 10 pg/mL
(MinDC+2SD; Leptin)
sensitivity: 15 pg/mL
(MinDC+2SD; Adiponectin)
sensitivity: 2.0 pg/mL
(MinDC+2SD; IL-1?)
sensitivity: 2.1 pg/mL
(MinDC+2SD; MCP-1)
sensitivity: 3.0 pg/mL
(MinDC+2SD; HGF)
sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)
sensitivity: 4.6 pg/mL
(MinDC+2SD; PAI-1 (total))
standard curve range: 0.29-4,000 pg/mL
(IL-8)
standard curve range: 0.32-5,000 pg/mL
(NGF)
standard curve range: 0.38-6,000 pg/mL
(TNFα)
standard curve range: 0.51-8,000 pg/mL
(IL-1β)
standard curve range: 0.96-150,000 pg/mL
(IL-6)
standard curve range: 0.96-150,000 pg/mL
(MCP-1)
standard curve range: 26-400,000 pg/mL
(Adipnectin)
standard curve range: 6.4-100,000 pg/mL
(HGF)
standard curve range: 6.4-100,000 pg/mL
(Resistin)
standard curve range: 9.6-150,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(PAI-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Adiponectin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Resistin)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)
inter-assay cv: <20%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <20%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(PAI-1 (total))
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)
inter-assay cv: <20%
intra-assay cv: <15%
(IL-1β)
inter-assay cv: <25%
intra-assay cv: <20%
(Leptin)

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

相关类别

一般描述

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack.

The MILLIPLEX® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

特异性

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

应用

  • Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
  • Recommended Sample Type: Tissue/cell culture supernatants or extracts
  • Recommended Sample Dilution: 25 μL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

特点和优势

Design your multiplex kit by choosing available analytes within this panel.

包装

Everything you need in a single kit.
96 well plate

储存及稳定性

Recommended storage for kit components is 2 - 8°C.

其他说明

Please contact Technical Service for linearity of dilution.

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Skull and crossbonesEnvironment
GHS06,GHS09

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Sonja Suvakov et al.
EBioMedicine, 70, 103536-103536 (2021-08-15)
Preeclampsia is a pregnancy-specific hypertensive disorder characterized by proteinuria and/or multisystem involvement. Disease-specific therapy has yet to be developed due to the lack of understanding of underlying mechanism(s). We postulate that accelerated ageing in general, and particularly cellular senescence, play
Pilar Codoñer-Franch et al.
Clinical biochemistry, 47(1-2), 44-48 (2013-11-05)
To evaluate whether serum resistin levels are related to cardiovascular risk in obese children. Cross-sectional study of 110 children (40 normal weight and 70 severely obese). Clinical and biochemical parameters, including lipid profile, fasting glucose and insulin, and homocysteine, were
Pamela J Goodwin et al.
Journal of the National Cancer Institute, 107(3) (2015-03-06)
Metformin may improve metabolic factors (insulin, glucose, leptin, highly sensitive C-reactive protein [hs-CRP]) associated with poor breast cancer outcomes. The NCIC Clinical Trials Group (NCIC CTG) MA.32 investigates effects of metformin vs placebo on invasive disease-free survival and other outcomes
Jing Zeng et al.
Endokrynologia Polska, 70(3), 248-254 (2019-03-08)
The primary objective of this study was to explore the association of pro-inflammatory cytokines with quantitative computed tomography (QCT)-measured bone mineral density (BMD) and its sexual difference in a Chinese population. In a cross-sectional study, a total of 85 participants
Cesar A Santa-Maria et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(12), 3024-3034 (2020-02-20)
We initiated a clinical trial to determine the proportion of breast cancer survivors achieving ≥5% weight loss using a remotely delivered weight loss intervention (POWER-remote) or a self-directed approach, and to determine the effects of the intervention on biomarkers of
查看所有相关文献

相关内容

Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门