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HADCYMAG-61K

Millipore

MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay

The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84

质量水平

200

种属反应性

human

制造商/商品名称

Milliplex®

assay range

accuracy: 100%
(IL-6)
accuracy: 102%
(TNFα)
accuracy: 103%
(MCP-1)
accuracy: 83%
(PAI-1 (total))
accuracy: 99%
(HGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; IL-8)
sensitivity: 0.2 pg/mL
(MinDC+2SD; NGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; TNF?)
sensitivity: 0.6 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 10 pg/mL
(MinDC+2SD; Leptin)
sensitivity: 15 pg/mL
(MinDC+2SD; Adiponectin)
sensitivity: 2.0 pg/mL
(MinDC+2SD; IL-1?)
sensitivity: 2.1 pg/mL
(MinDC+2SD; MCP-1)
sensitivity: 3.0 pg/mL
(MinDC+2SD; HGF)
sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)
sensitivity: 4.6 pg/mL
(MinDC+2SD; PAI-1 (total))
standard curve range: 0.29-4,000 pg/mL
(IL-8)
standard curve range: 0.32-5,000 pg/mL
(NGF)
standard curve range: 0.38-6,000 pg/mL
(TNFα)
standard curve range: 0.51-8,000 pg/mL
(IL-1β)
standard curve range: 0.96-150,000 pg/mL
(IL-6)
standard curve range: 0.96-150,000 pg/mL
(MCP-1)
standard curve range: 26-400,000 pg/mL
(Adipnectin)
standard curve range: 6.4-100,000 pg/mL
(HGF)
standard curve range: 6.4-100,000 pg/mL
(Resistin)
standard curve range: 9.6-150,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(PAI-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Adiponectin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Resistin)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)
inter-assay cv: <20%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <20%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(PAI-1 (total))
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)
inter-assay cv: <20%
intra-assay cv: <15%
(IL-1β)
inter-assay cv: <25%
intra-assay cv: <20%
(Leptin)

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

相关类别

一般描述

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack.

The MILLIPLEX® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

特异性

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

应用

  • Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
  • Recommended Sample Type: Tissue/cell culture supernatants or extracts
  • Recommended Sample Dilution: 25 μL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

特点和优势

Design your multiplex kit by choosing available analytes within this panel.

包装

Everything you need in a single kit.
96 well plate

储存及稳定性

Recommended storage for kit components is 2 - 8°C.

其他说明

Please contact Technical Service for linearity of dilution.

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Skull and crossbonesEnvironment
GHS06,GHS09

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

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Tracey McLaughlin et al.
Journal of the American Heart Association, 10(22), e021003-e021003 (2021-11-03)
Background Inflammation in epicardial adipose tissue (EAT) may contribute to coronary atherosclerosis. Myocardial bridge is a congenital anomaly in which the left anterior descending coronary artery takes a "tunneled" course under a bridge of myocardium: while atherosclerosis develops in the
Katharina Kessler et al.
Nutrients, 12(2) (2020-02-06)
: Meal timing affects metabolic regulation in humans. Most studies use blood samples for their investigations. Saliva, although easily available and non-invasive, seems to be rarely used for chrononutritional studies. In this pilot study, we tested if saliva samples could
Jelizaveta Sokolovska et al.
Physiological reports, 8(13), e14506-e14506 (2020-07-12)
Interval walking training has demonstrated more pronounced positive effects on physical fitness and metabolism in type 2 diabetes (T2D), compared to continuous walking. One of the pathogenic mechanisms of T2D is associated with derangements in leptin/adiponectin axis, which might predispose
Sonja Suvakov et al.
EBioMedicine, 70, 103536-103536 (2021-08-15)
Preeclampsia is a pregnancy-specific hypertensive disorder characterized by proteinuria and/or multisystem involvement. Disease-specific therapy has yet to be developed due to the lack of understanding of underlying mechanism(s). We postulate that accelerated ageing in general, and particularly cellular senescence, play
Pilar Codoñer-Franch et al.
Clinical biochemistry, 47(1-2), 44-48 (2013-11-05)
To evaluate whether serum resistin levels are related to cardiovascular risk in obese children. Cross-sectional study of 110 children (40 normal weight and 70 severely obese). Clinical and biochemical parameters, including lipid profile, fasting glucose and insulin, and homocysteine, were
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Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

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