推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
225, monoclonal
表单
lyophilized
不包含
preservative
种属反应性
human
请勿与下列物质发生反应
rat, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG1
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
一般描述
Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
免疫原
Human
partially purified, human EGFR from A431 cells
应用
Immunoprecipitation (1-2 µg per sample)
Neutralization Studies (2 µg/ml, see comments and application references)
Neutralization Studies (2 µg/ml, see comments and application references)
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
重悬
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
分析说明
Negative Control
HL-60 cells
HL-60 cells
Positive Control
A431 cells
A431 cells
其他说明
Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Pallavi Garg et al.
American journal of physiology. Lung cellular and molecular physiology, 301(1), L79-L90 (2011-05-03)
Thrombospondin-1 (TSP1) is a multidomain protein that contains epidermal growth factor (EGF)-like repeats that indirectly activate the EGF receptor (EGFR) and selected downstream signaling pathways. In these studies, we show that TSP1 opens the paracellular pathway in human lung microvascular
Anguo Liu et al.
The Journal of biological chemistry, 284(10), 6389-6402 (2009-01-09)
Thrombospondin (TSP) 1 is a trimeric multidomain protein that contains motifs that recognize distinct host cell receptors coupled to multiple signaling pathways. Selected TSP1-induced cellular responses are tyrosine kinase-dependent, and TSP1 contains epidermal growth factor (EGF)-like repeats. Specific receptor interactions
Silvia Ancona et al.
Biomedicines, 9(12) (2021-12-25)
Matrix metalloproteinase (MMP) dysregulation is implicated in several diseases, given their involvement in extracellular matrix degradation and cell motility. In lymphangioleiomyomatosis (LAM), a pulmonary rare disease, MMP-2 and MMP-9 have been detected at high levels in serum and urine. LAM
Caren C Bancroft et al.
International journal of cancer, 99(4), 538-548 (2002-05-07)
We previously reported that expression of angiogenesis factors interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) is promoted by coactivation of transcription factors nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) by interleukin-1alpha in human head and neck squamous cell carcinomas
Jiarui Bi et al.
Journal of cell science, 133(5) (2019-11-15)
In periodontal disease (PD), bacterial biofilms cause gingival inflammation, leading to bone loss. In healthy individuals, αvβ6 integrin in junctional epithelium maintains anti-inflammatory transforming growth factor-β1 (TGF-β1) signaling, whereas its expression is lost in individuals with PD. Bacterial biofilms suppress
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