Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14)

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.

This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).

K562 cells

Flow Cytometry (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Immunoblotting (not recommended)

Toxicity: Standard Handling (A)

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.

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Omary, M.B., et al. 1980. Nature286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.

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