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GF446

Sigma-Aldrich

HumKine® 热稳定bFGF,人重组

Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.

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别名:
FGF2, prostatropin, Prostatropin, HBGF-2, BFGF
UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.75

质量水平

检测方案

>95% (SDS-PAGE)

制造商/商品名称

HumanKine®

杂质

<0.5 μg/mg protein endotoxin (<5 EU/μg)

UniProt登记号

一般描述

与野生型bFGF蛋白相比,工程化的HumanKine Thermostable bFGF具有增强的热稳定性,而没有生物学功能的改变。

碱性成纤维细胞生长因子(bFGF)是一种肝素结合生长因子,可刺激包括间充质、神经外胚层和内皮细胞在内的多种细胞的增殖。bFGF在体内也具有强大的血管生成活性。人bFGF是一种含有154个氨基酸残基的17.2 kDa的蛋白。 bFGF可与BMP拮抗剂头蛋白协同作用,在无饲养层的条件下维持人胚胎干(hES)细胞的未分化增殖。
产品来源:HumanKine生长因子可在专有的人293蛋白表达系统中表达,在生产过程中未使用任何动物产品,并且被认为不含Xeno。

应用

HumanKine产品不含Xeno
与野生型bFGF蛋白相比,工程化的bFGF生长因子具有增强的热稳定性。使得对人类es/ips和神经干细胞培养的饲养条件不需要十分严格。
研究子范畴
生长因子 & 受体
研究类别
干细胞研究

质量

生物活性测定:通过剂量依赖性刺激3T3细胞增殖进行测定。ED50通常为≤0.5 ng/mL。

外形

从0.2 μm过滤的1X PBS溶液中冻干。

储存及稳定性

自收货之日起,在-20°C下最多保存4个月。应在添加了蛋白质的适当缓冲液或培养基中进行进一步稀释。

法律信息

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Qisheng Zuo et al.
EMBO reports, 23(1), e52491-e52491 (2021-11-09)
The unique characteristics of chicken primordial germ cells (PGCs) provide potential strategies for transgenic animal generation; however, insufficient PGC availability has limited their application. Regulation of bone morphogenic protein 4 (BMP4), a crucial factor for PGCs formation, may provide new
Fei Qian et al.
Molecular carcinogenesis, 58(4), 500-510 (2018-11-21)
HMGB1-RAGE signaling plays an integral role in inflammation-driven carcinogenesis. In the present study, we showed that RAGE has direct association with K-Ras following HMGB1 exposure in colorectal cancer (CRC) cells. Immunofluorescence analysis revealed a significant co-localization between RAGE and K-Ras
Qisheng Zuo et al.
Journal of cell science, 134(3) (2021-01-15)
The unique developmental characteristics of chicken primordial germ cells (PGCs) enable them to be used in recovery of endangered bird species, gene editing and the generation of transgenic birds, but the limited number of PGCs greatly limits their application. Studies
Megan Bowers et al.
Cell stem cell, 27(1), 98-109 (2020-05-11)
Altered neural stem/progenitor cell (NSPC) activity and neurodevelopmental defects are linked to intellectual disability. However, it remains unclear whether altered metabolism, a key regulator of NSPC activity, disrupts human neurogenesis and potentially contributes to cognitive defects. We investigated links between

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