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GF144

Sigma-Aldrich

人重组表皮生长因子蛋白

The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.

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别名:
EGF
UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.77

质量水平

检测方案

>98% (SDS-PAGE)

比活

≥1 x 10^7 U/mg

制造商/商品名称

Chemicon®

杂质

<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)

输入

sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)

NCBI登记号

UniProt登记号

运输

dry ice

一般描述

产品来源:大肠杆菌
表皮生长因子(EGF)是刺激多种表皮和上皮细胞增殖的多肽生长因子。人EGF是一个含有53个氨基酸残基的分子量为6.2 kDa的蛋白质。

应用

对于大多数体外应用,EGF在0.5至25.0 ng/mL的浓度范围内发挥其生物学活性。响应细胞包括(部分列表):表皮间皮细胞。

联系

替代:GF001

外形

从无菌过滤的10 mM磷酸钠(pH 7.5)冻干,不含添加剂。

储存及稳定性

冻干EGF尽管在室温下稳定,但最好在-20°C下干燥保存。 用水复溶成浓度为0.1 - 1 mg/mL。可以将该溶液稀释到其他缓冲溶液中,并在4°C下保存1周或-20°C以备将来使用。复溶的EGF应以未稀释的等分试样在-20°C下保存长达一年。

分析说明

比活度:与标准品相比,人EGF具有完全的生物活性。根据其对BALB/c 3T3细胞的剂量依赖性促有丝分裂活性确定的ED50为≤ 0.1 ng/mL

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK

WGK 3


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实验方案

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

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