产品名称
人重组表皮生长因子蛋白, The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.
assay
>98% (SDS-PAGE)
specific activity
≥1 x 10^7 U/mg
manufacturer/tradename
Chemicon®
impurities
<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)
input
sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Physical form
从无菌过滤的10 mM磷酸钠（pH 7.5）冻干，不含添加剂。
Preparation Note
冻干EGF尽管在室温下稳定，但最好在-20°C下干燥保存。 用水复溶成浓度为0.1 - 1 mg/mL。可以将该溶液稀释到其他缓冲溶液中，并在4°C下保存1周或-20°C以备将来使用。复溶的EGF应以未稀释的等分试样在-20°C下保存长达一年。
Analysis Note
比活度：与标准品相比，人EGF具有完全的生物活性。根据其对BALB/c 3T3细胞的剂量依赖性促有丝分裂活性确定的ED50为≤ 0.1 ng/mL
Application
对于大多数体外应用，EGF在0.5至25.0 ng/mL的浓度范围内发挥其生物学活性。响应细胞包括（部分列表）：表皮间皮细胞。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
产品来源：大肠杆菌
表皮生长因子（EGF）是刺激多种表皮和上皮细胞增殖的多肽生长因子。人EGF是一个含有53个氨基酸残基的分子量为6.2 kDa的蛋白质。
Other Notes
替代：GF001
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
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实验方案
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
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