产品名称
Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24), liquid, clone 5.24, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
5.24, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CSF3(1440)
Analysis Note
Positive Control
Recombinant G-CSF protein, bone marrow tissue, or fetal liver tissue
Recombinant G-CSF protein, bone marrow tissue, or fetal liver tissue
Application
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-Granulocyte Colony Stimulating Factor (Ab-1) mouse monoclonal, clone 5.24, recognizes ~19 kDa G-CSF in bone marrow & fetal liver tissue. It is validated for use in WB, IF & IHC (paraffin).
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes granulocyte colony stimulating factor.
Recognizes the ~19 kDa G-CSF glycoprotein in bone marrow and fetal liver tissue.
Immunogen
Human
purified human G-CSF
Other Notes
For paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Nicola, N.A. 1987. J. Cell Cloning5, 1.
Metcalf, D., 1986. Blood67, 257.
Nagata, S., et al. 1986. Nature319, 415.
Nagata, S., et al. 1986 EMBO J.5, 575.
Souza, L.M., et al. 1986 Science232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem.258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer26, 647.
Metcalf, D., 1986. Blood67, 257.
Nagata, S., et al. 1986. Nature319, 415.
Nagata, S., et al. 1986 EMBO J.5, 575.
Souza, L.M., et al. 1986 Science232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem.258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer26, 647.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Kazuhide Shimamatsu et al.
Oncology letters, 15(2), 2387-2392 (2018-02-13)
The present case study documents an autopsy case of granulocyte-colony stimulating factor (G-CSF)-producing mucinous cystic neoplasm (MCN), with an associated invasive carcinoma of the pancreas. A 65-year-old woman presented to Omuta City Hospital (Omuta Japan) with a primary complaint of
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