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Merck
CN
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文件

安全信息

ES-101

Sigma-Aldrich

含15% FBS和LIF的完整ES细胞培养基

Ready to use medium formulated with 15% fetal bovine serum (FBS) and mouse leukemia inhibitory factor (LIF). Each lot is qualified for mouse embryonic stem (ES) cell culture.

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UNSPSC代码:
12352207
eCl@ss:
32160801
NACRES:
NA.75

质量水平

400

形式

liquid

制造商/商品名称

Specialty Media

技术

cell culture | mammalian: suitable

输入

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

一般描述

含有15%FBS和LIF的完整胚胎干细胞培养基适用于与小鼠胚胎干细胞（ES）一起使用。它也可作为小鼠原代胚胎成纤维细胞（PMEF）饲养细胞生长的完整培养基。

应用

含有15%FBS和LIF的完整胚胎干细胞培养基已用于培养小鼠脑神经嵴细胞系（CNNC）。它还可用于培养小鼠胚胎干细胞。

外形

用于小鼠多能干（ES和iPS）细胞和饲养细胞培养的即用型培养基

储存及稳定性

储存于-20°C。 解冻后，培养基可在2-8°C下储存长达1个月。 避光保存。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK

WGK 1

法规信息

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Issei Saitoh et al.
Cell medicine, 3(1-3), 97-102 (2012-05-08)
Feeder cells are generally required for establishment and maintenance of embryonic stem (ES)/induced pluripotent stem (iPS) cells. Increased demands for generation of those cells carrying various types of vectors (i.e., KO vectors and transgenes) also require feeder cells that confer
Abijeet Singh Mehta et al.
Developmental biology, 471, 97-105 (2020-12-20)
During neurulation, cranial neural crest cells (CNCCs) migrate long distances from the neural tube to their terminal site of differentiation. The pathway traveled by the CNCCs defines the blueprint for craniofacial construction, abnormalities of which contribute to three-quarters of human
Akira Kinoshita et al.
Development (Cambridge, England), 148(16) (2021-08-03)
Mutations in ITPR1 cause ataxia and aniridia in individuals with Gillespie syndrome (GLSP). However, the pathogenic mechanisms underlying aniridia remain unclear. We identified a de novo GLSP mutation hotspot in the 3'-region of ITPR1 in five individuals with GLSP. Furthermore

实验方案

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

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