Antipain

Antipain is a serine and cysteine reversible protease inhibitor. It inhibits papain and trypsin. Plasmin is inhibited to a small extent as well. More specific than leupeptin for papain and trypsin.

Sequence:

[(S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-L-Arg-L-Val-Argininal-HCL-2H2O.

M.W: 604.71

Working solutions: 1-100μM.

Specific Activity: Varies with lot, IC50 is approximately 0.20μg/mL (papain/casein, pH 7.4 37°C).

Stable for several hours. Stock solutions of 10mM in water or buffer are stable for 1 week at 4ºC, 1 month at -20ºC. Also soluable in MeOH or DMSO.

5mg/827 microliters of water = 10mM solution. Dilute 1:100 for a 100μM solution.

Source: Microbial origin. Not synthetically made.

Proteinase inhibitor; Inhibits papain and trypsin. Plasmin is inhibited to a small extent.

Inhibitor Type: Protease Inhibitors

pH 1% aqueous solution: 5.2

Lyophilized no preservatives.

Properties: Soluble in H2O, methanol and DMSO (Stock solution: 10 mM). Stable at -20° C.

Mechanism of action: Formation of a hemicetal adduct between the aldehyde group of the inhibitor and the active serine of the proteinase.

Inhibition spectrum: Inhibits papain, trypsin and plasmin to a lesser extent. More specific for papain and trypsin than leupeptin. The inhibitory potency of antipain is 100-fold higher than that of elastatinal.

Pale yellow powder; material is isolated from cultured media of microorganisms. As with many of these compounds, they are too inherently unstable to allow for high standards of purity. The homogeneity of these compounds cannot be guaranteed except for their specific inhibitory activities.

Maintain dry at -20ºC for up to 18 months. Store reconstituted product in aliquots at -20ºC for up to 9 months. Do not thaw and refreeze.

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