生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1E4-G5, monoclonal
形式
liquid
不包含
preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NKX2-5(1482)
一般描述
Purified mouse monoclonal antibody. Recognizes the ~25-35 kDa NKX2-5 protein.
Recognizes the ~25-35 kDa NKX2-5 protein in HeLa cells and human thyroid tissue.
This Anti-NKX2-5 Mouse mAb (1E4-G5) is validated for use in ELISA, Immunoblotting for the detection of NKX2-5.
免疫原
Full-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein
应用
ELISA (see comments)
Immunoblotting (1-5 g/ml)
警告
Toxicity: Regulatory Review (Z)
外形
In PBS, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Negative Control
293T cells
293T cells
Positive Control
Human thyroid tissue, HeLa cells
Human thyroid tissue, HeLa cells
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Yadava, R,S., et al. 2008. Nat. Genet.40 61.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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