生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
不包含
preservative
种属反应性
monkey, rat, mouse, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer317/pSer317)
基因信息
human ... CHEK1(1111)
一般描述
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
应用
Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
重悬
Do not aliquot.
分析说明
Positive Control
Lysates from COS cells treated with UV
Lysates from COS cells treated with UV
其他说明
Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
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