推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
PAb416, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
SV40-infected cells
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
SV40 T Ag（Ab-2）是一种小鼠单克隆抗体，对SV40大T抗原特有的抗原决定簇具有特异性，与SV40小T抗原无反应性。两种抗原均由SV40基因组的早期区域编码。大的T抗原结合DNA，并与53,000道尔顿的细胞转化蛋白p53形成复合物，p53是裂解生长过程中启动病毒DNA复制所必需的。此外，大T抗原与DNA聚合酶和转录因子AP-2结合，并与视网膜母细胞瘤易感基因的p105产物形成特异性复合物。
识别SV80和SV-T2细胞中的~94 kDa SV40大T抗原。
该抗SV40 T抗原（Ab-2）小鼠mAb（PAb416）经验证可用于亲和纯化、免疫印迹、IF、IP和石蜡切片，以检测SV40 T抗原（Ab-2）。
通过用指定的免疫原免疫小鼠并将脾细胞与NS-1小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体（请参阅应用参考）。识别~94 kDa SV40大T抗原。
免疫原
纯化SV40大T抗原
表位：氨基酸83-128内
应用
亲和纯化（使用目录编号DP02A）
免疫印迹（1-5 µg/ml，请参阅应用参考）
免疫荧光（1-5 µg/ml）
免疫沉淀（1-5 µg抗体，使用目录编号DP02A）
石蜡切片（请参阅应用参考）
免疫印迹（1-5 µg/ml，请参阅应用参考）
免疫荧光（1-5 µg/ml）
免疫沉淀（1-5 µg抗体，使用目录编号DP02A）
石蜡切片（请参阅应用参考）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
溶于50 mM磷酸钠缓冲液（pH 7.5），含0.2％明胶。
分析说明
阳性对照
SV80或SV-T2细胞
SV80或SV-T2细胞
阴性对照
3T3细胞
3T3细胞
其他说明
DeCaprio, J.A., et al. 1988.Cell54, 275.
Whyte, P., et al. 1988.Nature334, 124.
Mitchell, P.J., et al. 1987.Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985.J. Virol.53, 1001.
Simanis, V. and Lane, D.P.1985.Virol.144, 88.
Mann, R.S. and Carroll, R.B.1984.Virology138, 379.
Sarnow, P., et al. 1982.Cell28, 387.
Crawford, L.V., et al. 1981.Proc.Natl.Acad.Sci. USA78, 41.
Lane, D.P. and Crawford, L.V.1979.Nature278, 261.
Carroll, R.B., et al. 1974.Proc.Natl.Acad.Sci. USA71, 3754.
Tooze, J. 1973.Cold Spring Harbor, New York.
Tegtmeyer, P. 1972.J. Virol.10, 591.
Whyte, P., et al. 1988.Nature334, 124.
Mitchell, P.J., et al. 1987.Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985.J. Virol.53, 1001.
Simanis, V. and Lane, D.P.1985.Virol.144, 88.
Mann, R.S. and Carroll, R.B.1984.Virology138, 379.
Sarnow, P., et al. 1982.Cell28, 387.
Crawford, L.V., et al. 1981.Proc.Natl.Acad.Sci. USA78, 41.
Lane, D.P. and Crawford, L.V.1979.Nature278, 261.
Carroll, R.B., et al. 1974.Proc.Natl.Acad.Sci. USA71, 3754.
Tooze, J. 1973.Cold Spring Harbor, New York.
Tegtmeyer, P. 1972.J. Virol.10, 591.
也可用琼脂糖偶联物（目录编号DP02A），适用于亲和纯化和免疫沉淀。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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