推荐产品
生物来源
goat
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
lyophilized
不包含
preservative
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
免疫亲和纯化的山羊多克隆抗体，吸附人血清蛋白并与辣根过氧化物酶偶联。可识别兔IgG。
经验证，该山羊抗兔IgG过氧化物酶偶联物可用于ELISA和免疫组化以检测兔IgG。
应用
ELISA（1:5000-1:100,000）
免疫印迹（1:5000-1:100,000，比色；1:10,000-1:200,000，化学发光）
免疫组织化学（1:500-1:5000）
免疫印迹（1:5000-1:100,000，比色；1:10,000-1:200,000，化学发光）
免疫组织化学（1:500-1:5000）
警告
毒性：标准处理（A）
外形
从250 mM NaCl，10 mM磷酸钠，15 mg/ml BSA（pH 7.6）中冻干。
重悬
用1.5 ml H₂O复溶抗体。由于充分混合会使抗体变性，因此在4°C下轻轻混合使其溶解。在复溶后分装并冷冻保存（-70°C）。避免溶液反复冻融。或者，可以添加甘油（最终浓度为50%），然后将复溶的抗体储存于-20°C。请注意，在这些条件下，抗体和缓冲盐的浓度将为原始浓度的一半。
其他说明
为了确保这些试剂的质量，每个批次都使用我们的几种一抗进行测试。通过免疫电泳检测抗体特异性，结果显示对兔IgG和大多数兔免疫球蛋白常见的轻链具有特异性。未检测到与非免疫球蛋白血清蛋白的交叉反应性。该抗体也已通过ELISA和/或吸附进行了检测，以确保与人血清蛋白的交叉反应性降至最低，但该抗体可能与其他物种的免疫球蛋白具有交叉反应性。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
Mayara Caldas Ramos Cunha et al.
PloS one, 8(3), e58872-e58872 (2013-03-22)
Protein malnutrition (PM) results in pathological changes that are associated with peripheral leukopenia, bone marrow (BM) hypoplasia and alterations in the BM microenvironment leading to hematopoietic failure; however, the mechanisms involved are poorly understood. In this context, the BM mesenchymal
Karl A Nath et al.
The American journal of pathology, 166(4), 963-972 (2005-03-29)
Ischemic injury is invoked as a mechanism contributing to end-organ damage and other complications of sickle cell disease (SCD). However, the intrinsic sensitivity of tissues in SCD to ischemic insults has never been addressed. We examined the effect of renal
Luba Sominsky et al.
Frontiers in neuroscience, 7, 100-100 (2013-06-20)
Neonatal immune challenge by administration of lipopolysaccharide (LPS) produces enduring alterations in the development and activity of neuroendocrine, immune and other physiological systems. We have recently reported that neonatal exposure to an immune challenge by administration of LPS results in
Bettina P Mihalas et al.
Aging, 11(10), 2968-2997 (2019-05-28)
Oocytes are reliant on messenger RNA (mRNA) stores to support their survival and integrity during a protracted period of transcriptional dormancy as they await ovulation. Oocytes are, however, known to experience an age-associated alteration in mRNA transcript abundance, a phenomenon
Chih-Cheng Lin et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(8), 3300-3309 (2019-02-07)
The rice SUB1A-1 gene, which encodes a group VII ethylene response factor (ERFVII), plays a pivotal role in rice survival under flooding stress, as well as other abiotic stresses. In Arabidopsis, five ERFVII factors play roles in regulating hypoxic responses.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门