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一般描述
蛋白G纯化的小鼠单克隆抗体，通过用指定的免疫原免疫BALB/c小鼠，并将脾细胞与小鼠骨髓瘤SP2/0细胞融合而产生。识别~130（纽蛋白）和~150 kDa（间纽蛋白）蛋白。
识别皮肤组织中的~130（纽蛋白）和~150 kDa（间纽蛋白）蛋白。
该抗-纽蛋白小鼠mAb（V284或VLN01）经验证可用于免疫印迹，免疫沉淀，石蜡切片以检测纽蛋白。
免疫原
人
半纯化人纽蛋白
应用
免疫印迹法（1 g/mL，化学发光法）
免疫沉淀（2 g/mL裂解物）
石蜡切片（4-8 g/ml，需要加热预处理）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
在10 mM PBS，0.2%BSA，pH值7.4中。
分析说明
阳性对照
皮肤细胞或组织
皮肤细胞或组织
阴性对照
正常小鼠血清
正常小鼠血清
其他说明
Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol.79, 961.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J.1990 Cell Motil.Cytoskeleton16, 1.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J.1990 Cell Motil.Cytoskeleton16, 1.
该抗体可用于研究纽蛋白在血小板活化中的作用。当用于免疫组化时，纽蛋白（Ab-1）表现出膜和细胞质染色。对于福尔马林固定石蜡切片的染色，在10 mM柠檬酸盐缓冲液（pH 6.0）中煮沸10-20分钟，然后在室温下冷却20分钟。应滴定抗体，以在单个系统中获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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