推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
FBN11, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rat, human, mouse
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)
一般描述
Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
免疫原
recombinant human fibronectin
应用
Frozen Sections (0.5-1 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
分析说明
Positive Control
SW 156 cells or normal kidney tissue
SW 156 cells or normal kidney tissue
其他说明
Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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