推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
X22, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
bovine, mouse, rat, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CLTCL1(8218)
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
Recognizes the ~180 kDa clathrin heavy chain protein.
This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.
免疫原
Human
clathrin heavy chain purified from human brain
应用
Immunoblotting (1:500)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation (see comments)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation (see comments)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In PBS.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Bauerfeind, R., et al. 1996. J. Neurocytol.25, 701.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.
Immunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Dinah Loerke et al.
Traffic (Copenhagen, Denmark), 6(10), 918-929 (2005-09-06)
The clathrin triskelion is composed of three light chain (LC) and three heavy chain (HC) subunits. Cellular control of clathrin function is thought to be aimed at the LC subunit, mainly on the basis of structural information. To test this
Anna V Salova et al.
Oncotarget, 7(5), 6029-6047 (2015-12-31)
EGF complexed to fluorescent photostable quantum dots by biotin-streptavidin system (bEGF-savQD) is attractive for both the basic research and therapeutic application such as targeted drug delivery in EGF-receptor (EGFR) expressing cancers. However, compared to native EGF, the large size of
AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine.
Vandana Kumari et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 16(3), 327-334 (2017-04-26)
AP2 is a clathrin-based endocytic adaptor complex comprising α, β2, μ2 and σ2 subunits. μ2 regulates CFTR endocytosis. The α subunit interacts with CFTR in the intestine but its physiologic significance is unclear. CFTR short circuit current was measured in
Stephanie A Zlatic et al.
Molecular biology of the cell, 24(15), 2378-2388 (2013-06-14)
A role for clathrin in AP-3-dependent vesicle biogenesis has been inferred from biochemical interactions and colocalization between this adaptor and clathrin. The functionality of these molecular associations, however, is controversial. We comprehensively explore the role of clathrin in AP-3-dependent vesicle
April Thelemann et al.
Molecular & cellular proteomics : MCP, 4(4), 356-376 (2005-01-20)
Overexpression and enhanced activation of the epidermal growth factor (EGF) receptor are frequent events in human cancers that correlate with poor prognosis. Anti-phosphotyrosine and anti-EGFr affinity chromatography, isotope-coded muLC-MS/MS, and immunoblot methods were combined to describe and measure signaling networks
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