一般描述
抗CDH1(Ab-2),小鼠单克隆,克隆DH01,可识别大鼠1细胞中约55 kDa Cdh-1蛋白。它已被验证可用于蛋白质印迹和免疫沉淀。
用指定的免疫原免疫BALB/c小鼠,并将脾细胞与Sp2/0小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。识别约为55 kDa的ID1蛋白。
识别大鼠1细胞中~55 kDa Cdh-1蛋白。
免疫原
人
重组人Cdh1蛋白
应用
免疫印迹(1 g/mL)
免疫沉淀(2 g/mg蛋白裂解物)
包装
请参考特定浓度批号的标签。
警告
毒性:标准处理(A)
外形
在10 mM PBS,0.2%BSA,pH值7.4中。
分析说明
阳性对照
:KAT或LS174T细胞
:KAT或LS174T细胞
阴性对照
正常小鼠血清
正常小鼠血清
其他说明
Kramer, E.R., et al. 2000 Mol.Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol.Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc.Natl.Acad.Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol.Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc.Natl.Acad.Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.
不与其他含有WD重复序列的蛋白质(包括Cdc20)发生交叉反应。在单个系统中,应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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