CC095-M
Mouse Laminin
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, liquid, 1 mg/mL, suitable for cell culture
别名:
从小鼠中提取的纯化层粘连蛋白
产品名称
层粘连蛋白，小鼠纯化, Laminins are the major noncollagenous constituent of basement membranes. This purified mouse laminin was isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a mouse tumor that produces large amounts of basement membranes.
biological source
mouse
form
liquid
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
input
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Application
Biochem/physiol Actions
Disclaimer
General description
Physical form
无防腐剂。
Preparation Note
在2-8°C下小心解冻产品，以避免过早凝胶化，请勿重新冷冻。解冻后，物料应在2-8°C下储存长达3个月。
制备：根据Templ et al 1979 {http：//www.jbc.org/cgi/reprint/254/19/9933.pdf}的方法从Engelbreth-Holm-Swarm{EHS}肉瘤制备简单地说，对肿瘤匀浆进行NaCl提取和离心。在0.5 M NaCl馏分中发现了层粘连蛋白。在随后的盐梯度和进一步的离心步骤之后，将层粘连蛋白用2M尿素透析，并通过DEAE柱，然后进行分子筛层析和超滤浓缩。
Legal Information
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。
实验方案
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。
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