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CBL218

Sigma-Aldrich

Anti-Keratin K3/K76 抗体,克隆 AE5

clone AE5, Chemicon®, from mouse

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别名:
Anti-HUMCYT2A, Anti-KRT2P
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

AE5, monoclonal

种属反应性

human, mouse, bovine, rabbit

制造商/商品名称

Chemicon®

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... KRT3(3850)

特异性

该抗体与角膜上皮的64 kDa多肽(细胞角蛋白3;角蛋白K3)和上颚上皮的角蛋白K76(细胞角蛋白K2p)反应。 因此,这代表了研究角膜上皮类型分化的极佳标记。也可识别牛口鼻和唇上皮以及兔角膜细胞系。

免疫原

兔角膜上皮角蛋白

应用

使用冷冻切片进行的免疫组化:1:50。

使用石蜡包埋切片进行的免疫组化:在胰蛋白酶消化抗原修复后,将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中(Mikaelian et al.,2004)。

蛋白质印迹:1:500。

最佳工作稀释度必须由最终用户进行确定。
抗角蛋白K3/K76抗体,克隆AE5是抗角蛋白K3/K76的抗体,用于WB,IH,IH(P)。

目标描述

64 kDa

外形

形式:纯化
通过离子交换色谱法纯化单克隆抗体,将其置于含有0.09%叠氮化钠的磷酸盐缓冲生理盐水(pH 7.2)中。

储存及稳定性

若购买后1个月内使用,则储存于2°-8°C,若要长期储存,则将抗体等分成小体积并储存于-20°C,保存达1年。

其他说明

浓度:请参考批次特异性浓缩物的检验报告。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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