产品名称
Anti-Keratin K3/K76 抗体，克隆 AE5, clone AE5, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AE5, monoclonal
species reactivity
human, mouse, bovine, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KRT3(3850)
Application
使用冷冻切片进行的免疫组化：1:50。
使用石蜡包埋切片进行的免疫组化：在胰蛋白酶消化抗原修复后，将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中（Mikaelian et al.，2004）。
蛋白质印迹：1:500。
最佳工作稀释度必须由最终用户进行确定。
使用石蜡包埋切片进行的免疫组化：在胰蛋白酶消化抗原修复后，将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中（Mikaelian et al.，2004）。
蛋白质印迹：1:500。
最佳工作稀释度必须由最终用户进行确定。
抗角蛋白K3/K76抗体，克隆AE5是抗角蛋白K3/K76的抗体，用于WB，IH，IH（P）。
Biochem/physiol Actions
该抗体与角膜上皮的64 kDa多肽（细胞角蛋白3；角蛋白K3）和上颚上皮的角蛋白K76（细胞角蛋白K2p）反应。 因此，这代表了研究角膜上皮类型分化的极佳标记。也可识别牛口鼻和唇上皮以及兔角膜细胞系。
General description
64 kDa
Immunogen
兔角膜上皮角蛋白
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
通过离子交换色谱法纯化单克隆抗体，将其置于含有0.09%叠氮化钠的磷酸盐缓冲生理盐水（pH 7.2）中。
Preparation Note
若购买后1个月内使用，则储存于2°-8°C，若要长期储存，则将抗体等分成小体积并储存于-20°C，保存达1年。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Fiona J Li et al.
Experimental eye research, 147, 1-11 (2016-04-18)
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The transplantation of expansions of limbal epithelial stem cells (LESC) remains one of the most efficient therapies for the treatment of limbal stem cell deficiency (LSCD) to date. However, the available donor corneas are scarce, and the corneas conserved for
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