推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
IT-Ks20.8, monoclonal
种属反应性
human
请勿与下列物质发生反应
mouse, rat
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG2a
NCBI登记号
UniProt登记号
运输
wet ice
基因信息
human ... KRT20(54474)
mouse ... Krt20(66809)
rat ... Krt20(286912)
一般描述
IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
特异性
The antibody is specific for the 46 kDa polypeptide (cytokeratin 20).
免疫原
Electrophoretically purified cytokeratin 20 from human intestinal mucosa
应用
Western blotting
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.
Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
联系
Replaces: MABT150
外形
Format: Purified
Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.
储存及稳定性
Once reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles.
分析说明
Control
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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危险声明
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
储存分类代码
11 - Combustible Solids
WGK
WGK 3
P Harnden et al.
British journal of urology, 78(6), 870-875 (1996-12-01)
To investigate the dysregulation of cytokeratin 20 (CK20) expression in urothelial dysplasia and its potential as a diagnostic aid. Twenty-two patients were selected on the basis that they had undergone one or more biopsies showing dysplasia before the development of
Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins.
Demirkesen, C, et al.
Journal of cutaneous pathology, 22, 518-535 (1995)
The distribution and origin of keratin 20-containing taste buds in rat and human.
Zhang, C and Oakley, B
Differentiation, 61, 121-127 (1996)
V J Möbus et al.
British journal of cancer, 69(3), 422-428 (1994-03-01)
Two human ovarian (OV-MZ-10, OV-MZ-15) and two colon cancer cell lines (CO-MZ-5, CO-MZ-6) were newly established in permanent cell culture. These cell lines have been maintained in vitro for 5-6 years, the passage number varying from 25 to 228. They
I Moll et al.
Archives of dermatological research, 283(5), 300-309 (1991-01-01)
Human eccrine sweat gland ducts and benign and malignant eccrine poromas were studied for the expression of various cytokeratins (CK) and vimentin by applying immunoperoxidase and immunofluorescence microscopy to frozen or paraffin-embedded sections, and using two-dimensional gel electrophoresis and immunoblotting.
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