Anti-Actin Antibody, smooth muscle, clone ASM-1

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.

Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.

This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).

Epitope: N-terminus

Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.

Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.

Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.

Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.

43 kDa

Replaces: 04-1040

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

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