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用途
sufficient for 96 tests
质量水平
包装
pkg of 1 96-well plate(s)
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
输入
sample type monocyte(s)
检测方法
fluorometric
运输
wet ice
一般描述
The InnoCyte Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.
组分
96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.
警告
Toxicity: Irritant (B)
原理
The Calbiochem InnoCyte 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.
制备说明
Cell Labeling Mixture: Warm the D-PBS and Calcein-AM Solution to room temperature. Add 35 µl Calcein-AM Solution to 10 ml D-PBS. Mix well. Note: Prepare just before use (see step 7 of the Detailed Protocol below).
储存及稳定性
Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
其他说明
Cravens, P.D. 2007. Scan. J. Immunol.65, 514.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10-13 - German Storage Class 10 to 13
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