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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2D7, monoclonal
形式
liquid
不包含
preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG2a
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NDRG1(10397)
一般描述
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.
Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.
This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.
免疫原
Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein
应用
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (0.7 g/ml)
警告
Toxicity: Regulatory Review (Z)
外形
In PBS, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Human endometrium tissue, HeLa cells
Human endometrium tissue, HeLa cells
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Jiang, K., et al. 2010. Scand. J. Gastroenterol45, 898.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Lianghui Dong et al.
PLoS pathogens, 17(6), e1009645-e1009645 (2021-06-03)
The presumed DNA helicase encoded by ORF44 of Kaposi's sarcoma-associated herpesvirus (KSHV) plays a crucial role in unwinding viral double-stranded DNA and initiating DNA replication during lytic reactivation. However, the regulatory mechanism of KSHV ORF44 has not been fully elucidated.
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