推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer300)
一般描述
免疫亲和纯化的兔多克隆抗体。可识别出在Ser300处磷酸化的~44 kDa的PDH-E1α蛋白。
识别HEK293细胞中在Ser300磷酸化的~44 kDa PDH-E1α蛋白。
该PhosphoDetect抗-PDH-E1α pSer³⁰⁰）兔pAb经验证可用于免疫印迹法、免疫细胞化学检测PDH-E1α（pSer³⁰⁰）。
免疫原
人
合成磷酸肽，对应于人PDH-E1的Ser³⁰⁰磷酸化位点周围的氨基酸α
应用
免疫印迹（0.5μg/ml）
免疫细胞化学（1μg/ml）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
在150 mM NaCl，100 mM Tris-甘氨酸，pH 7.4中。
重悬
初次融化后，等分并冷冻（-20°C）。
分析说明
对照
HEK293细胞
HEK293细胞
阴性对照
用二氯乙酸盐处理的HEK293细胞
用二氯乙酸盐处理的HEK293细胞
其他说明
Rardin M.J., et. al. 2009.Anal.Biochem.2, 157.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
由于高程度序列一致性，该抗体也预期与小鼠，大鼠，牛，斑马鱼和非洲爪蟾发生交叉反应。磷酸化PDH-E1α抗体应进行滴定以在单个系统中获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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