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Merck
CN

AM80

抗-PAR(Ab-1)小鼠mAb(10H)

liquid, ≥95% (SDS-PAGE), clone 10H

别名:

抗多腺苷二磷酸核糖

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
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产品名称

抗-PAR(Ab-1)小鼠mAb(10H), liquid, ≥95% (SDS-PAGE), clone 10H

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

10H, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, Drosophila, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (2.5 µg/mL)
Immunocytochemistry (5-20 µg/mL))

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Analysis Note

阳性对照
依托泊苷处理的HL-60细胞
阴性对照
正常小鼠IgG

Application

免疫印迹(2.5 µg/ml)

免疫细胞化学(5-20 µg/ml)

Disclaimer

毒性:标准处理(A)

General description

蛋白 A 纯化的小鼠单克隆抗体。识别聚ADP-核糖。
识别依托泊苷处理的HL-60细胞中的聚(ADP)-核糖(PAR)。
该抗PAR(Ab-1)小鼠mAb(10H)经验证可用于免疫印迹、免疫细胞化学检测PAR(Ab-1)。

Immunogen

纯化的聚(ADP-核糖)

Other Notes

Kawamitsu, H., et al. 1984.Biochemistry23, 3771.
用67 µM依托泊苷处理3.5 h可诱导HL-60细胞中的PAR。应滴定抗体,以在单个系统中获得最佳结果。

Packaging

请参考特定浓度批号的标签。

Physical form

在100 mM NaCl,50 mM HEPES,1% BSA,pH 7.4中。

Preparation Note

初次融化后,等分并冷冻(-20°C)。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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