生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
10H, monoclonal
检测方案
≥95% (SDS-PAGE)
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, Drosophila, rat, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG3
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
蛋白 A 纯化的小鼠单克隆抗体。识别聚ADP-核糖。
识别依托泊苷处理的HL-60细胞中的聚(ADP)-核糖(PAR)。
该抗PAR(Ab-1)小鼠mAb(10H)经验证可用于免疫印迹、免疫细胞化学检测PAR(Ab-1)。
免疫原
牛
纯化的聚(ADP-核糖)
应用
免疫印迹(2.5 µg/ml)
免疫细胞化学(5-20 µg/ml)
免疫细胞化学(5-20 µg/ml)
包装
请参考特定浓度批号的标签。
警告
毒性:标准处理(A)
外形
在100 mM NaCl,50 mM HEPES,1% BSA,pH 7.4中。
重悬
初次融化后,等分并冷冻(-20°C)。
分析说明
阳性对照
依托泊苷处理的HL-60细胞
依托泊苷处理的HL-60细胞
阴性对照
正常小鼠IgG
正常小鼠IgG
其他说明
Kawamitsu, H., et al. 1984.Biochemistry23, 3771.
用67 µM依托泊苷处理3.5 h可诱导HL-60细胞中的PAR。应滴定抗体,以在单个系统中获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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