Anti-TGN46, Antibody

TGN46 is one of several proteins that utilize retrograde transport to travel between the trans-Golgi network (TGN) and the endocytic pathway. Retrograde transport involves the tethering, docking, fusion, and post translational modification of TGN proteins. Further studies are still required to elucidate the roles and mode of action for TGN46.

This antibody recognizes the cytoplasmic domain of TGN46.

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TGN46.

Anti-TGN46 detects levels of TGN46 proteins & has been published & validated for use in wb, IP & ICC.

Research Category
Cell Structure

Research Sub Category
Vesicular Trafficking

Western Blot Analysis: 2 µg/mL of this antibody detected TGN46 in 10 µg of NIH/3T3 cell lysate. Multiple uncharacterized bands may be observed in some cell lysates.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TGN46 in A431 and HeLa cells.

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 0.2 µg of this antibody immunoprecipitated TGN46 in 500 µg of HeLa cell lysate (An uncharacterized band at ~29 kDa may be observed in some cell lysates.).

~110 kDa observed. This protein is observed at ~110 kDa, and it is subject to glycosylation (Khanobdee, K. et al. (2004). Mol Vis. 13(10):933-42.; Prescott, A. R., et al. (1997). Eur J Cell Biol. 72(3):238-246.).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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