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生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse, human
种属反应性(根据同源性预测)
rat (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), canine (based on 100% sequence homology)
技术
immunocytochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ARPIN(348110)
一般描述
Arpin (UniProt Q7Z6K5; also known as Arp2/3 inhibition protein, UPF0552 protein C15orf38) is encoded by the ARPIN (also known as C15orf38) gene (Gene ID 348110) in human. Cell migration requires the generation of branched actin networks that power the protrusion of the plasma membrane in lamellipodia. The actin-related proteins 2 and 3 (Arp2/3) complex nucleates actin filaments by binding to the side of an existing filament and initiating branch formation. Arpin functions as a negative regulator of Arp2/3 activity, allowing cells to control directional persistence of migration by fine-tuning actin nucleation activity at the leading edge of the lamellipodium. Arpin depletion in both mammalian cells and Dictyostelium discoideum amoeba is reported to result in straighter trajectories. Arpin microinjection is demonstrated to induce fish keratocytes to turn.
特异性
Reacts with both Arpin and the C15orf38-AP3S2 fusion (isoform 2) produced from a naturally occurring readthrough transcript.
免疫原
Epitope: near Arpin C-terminus
KLH-conjugated linear peptide corresponding to human Arpin near the C-terminus.
应用
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected detected Arpin and C15orf38-AP3S2 in MEF-1 cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-Arpin Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of Arpin.
质量
Evaluated by Western Blotting in MEF-1 murine embryonic fibroblasts cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected detected Arpin and C15orf38-AP3S2 in 10 µg of human U2OS and murine MEF-1 embryonic fibroblasts cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected detected Arpin and C15orf38-AP3S2 in 10 µg of human U2OS and murine MEF-1 embryonic fibroblasts cell lysate.
目标描述
~28/42 kDa observed (24.9 kDa/Arpin and 43.9 kDa/C15orf38-AP3S2 calculated)
外形
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
The Journal of experimental medicine, 214(10), 2889-2900 (2017-08-23)
Epithelial carcinomas are well known to activate a prolonged wound-healing program that promotes malignant transformation. Wound closure requires the activation of keratinocyte migration via a dual-state molecular switch. This switch involves production of either the anti-migratory microRNA miR-198 or the
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