生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性(根据同源性预测)
primate (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), feline (based on 100% sequence homology), monkey (based on 100% sequence homology)
技术
immunohistochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... LGALS4(3960)
一般描述
Galectin 4, or GAL-4, is another member of the Galectin family. Galectins are carbohydrate binding proteins that appear important in cell-cell contact, cell migration, and cell to cell signaling in both normal and disease (cancerous) tissues. Galectin 4 differs from its sister family members in the range of carbohydrate substrates that it binds. Galentin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine. It is often found in relatively insoluble complexes as a component of either adherent junctions or lipid rafts in the microvillus membrane, and it has been proposed to stabilize these structures. Strong expression of galectin-4 can be induced, however, in cancers from other tissues including breast and liver. Also, Galectin-4 appears to specifically bind CD3 on activated T-cells in the gut and regulate their activity via calpain mediated apoptosis.
免疫原
Epitope: near N-terminal
KLH-conjugated linear peptide corresponding to human Galectin-4 near the N-terminal.
应用
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-Galectin-4 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Galectin-4.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of Colo320 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of human small intestine tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Galectin-4 in human stomach tissue.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of human small intestine tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Galectin-4 in human stomach tissue.
质量
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Galectin-4 in 10 µg of HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Galectin-4 in 10 µg of HT-29 cell lysate.
目标描述
~36 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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