Anti-TRPM8 Antibody

Transient receptor potential cation channel subfamily M member 8 (TRPM8) is also known as Long transient receptor potential channel 6 (LTrpC-6), and Transient receptor potential p8 (Trp-p8). TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness. TRPM8 is activated by icilin, eucalyptol, menthol, cold and changes to intracellular pH. TRPM8 is expressed in prostate as well as tumors of the prostate, colon, lung, breast and skin. TRPM8 expression in most prostate tumors and the presence of an immunogenic epitope suggest that it may be suitable for the design of peptide vaccination strategies for prostate cancers.

Other homologies: Bovine (88% sequence homology), Canine (71% sequence homology), and Mouse and Rat (53% sequence homology).

This antibody recognizes the cytoplasmic domain of TRPM8. This antibody will recognize isoform 1.

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TRPM8.

Detect TRPM8 using this rabbit polyclonal antibody, Anti-TRPM8 Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRPM8 in human prostate cancer and breast cancer cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected TRPM8 in 10 µg of LNCaP cell lysate.

~115 kDa observed

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
LNCaP cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.