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主要文件

ABT146

Sigma-Aldrich

抗闭合蛋白抗体

from rabbit, purified by affinity chromatography

别名:

Occludin

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25 μG
CN¥1,455.13
100 μG
CN¥4,383.64

CN¥1,455.13

预计发货时间May 12, 2025详情

针对您的靶标提供了无防腐剂重组抗体。欢迎选购 ZRB1451
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25 μG
CN¥1,455.13
100 μG
CN¥4,383.64

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥1,455.13

预计发货时间May 12, 2025详情

针对您的靶标提供了无防腐剂重组抗体。欢迎选购 ZRB1451
登录查看协议价
获取大包装报价

生物来源

rabbit

质量水平

100

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human

种属反应性（根据同源性预测）

bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), primate (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology)

包装

antibody small pack of 25 μg

技术

western blot: suitable

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此商品
SAB4200593SAB5700784HPA005933
Anti-Occludin Antibody from rabbit, purified by affinity chromatography

ABT146

Anti-Occludin Antibody

Anti-Occludin antibody from rabbit affinity isolated antibody

SAB4200593

Anti-Occludin antibody from rabbit

Anti-Occludin antibody produced in rabbit

SAB5700784

Anti-Occludin antibody produced in rabbit

Anti-OCLN antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

HPA005933

Anti-OCLN antibody produced in rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

UniProt accession no.

Q16625

UniProt accession no.

Q16625

UniProt accession no.

Q16625

UniProt accession no.

Q16625

species reactivity

human

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

human

technique(s)

western blot: suitable

technique(s)

immunoblotting: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

immunofluorescence: 1:50-1:100, immunohistochemistry: 1:50-1:200, western blot: 1:1000-1:2000

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500

一般描述

闭锁蛋白是位于紧密连接的 65 kDa 完整的血浆膜蛋白。它是细胞因子诱导的紧密连接的细胞旁渗透屏障调节所必需的。人们相信闭锁蛋白阻止大分子通过紧密连接的运输过程。闭锁蛋白由 OLCN 基因编码，横跨质膜四次，形成两个胞外环，并将其 NH2 和 COOH 末端暴露于胞质中。该胞质尾巴直接结合至 ZO 家族蛋白，该家族将蛋白复合物连接至肌动蛋白细胞骨架。磷酸化/去磷酸化在调节闭锁蛋白和紧密连接中起主要作用。闭锁蛋白的表达是丙型肝炎病毒（HCV）感染肝细胞所必需的。

特异性

该抗体识别闭锁蛋白的 N 端。

免疫原

与人封闭蛋白 N 末端相对应的 KLH 偶联的线性肽。
表位：N端

应用

研究子类别
ECM 蛋白
研究类别
细胞结构
该抗封闭蛋白抗体经验证可用于蛋白质印迹法检测封闭蛋白。

质量

通过蛋白质印迹在人肾组织裂解液中进行了评估。

蛋白质印迹分析：0.2 µg/mL 的该抗体在 10 µg 的人肾脏组织裂解液中检测到了封闭蛋白。

目标描述

观测值〜63 kDa。计算出的分子量为 63 kDa，但是封闭蛋白在蛋白质印迹中的观察到的分子量范围为 ~55 至 ~65 kDa（Florian, P., et al. (2003).Biochemical and Biophysical Research Communications.304:5-10）。

外形

亲和纯化
在含 0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。

储存及稳定性

自收到之日起，在 2-8°C 条下可稳定保存 1 年。

分析说明

对照
人肾组织裂解液

其他说明

浓度：请参考批次特异性浓缩物的分析证书。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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Blood-brain barrier (BBB) disruption is a common feature in neurodegenerative diseases. However, BBB integrity has not been assessed in spinocerebellar ataxias (SCAs) such as Machado-Joseph disease/SCA type 3 (MJD/SCA3), a genetic disorder, triggered by polyglutamine-expanded ataxin-3. To investigate that, BBB
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Journal of leukocyte biology, 111(5), 953-966 (2021-09-30)
Ischemic stroke (IS) can cause disability and death, and microglia as the immune component of the CNS can release inflammatory factors and participate in blood-brain barrier (BBB) dysfunction. This study aimed to investigate the effects of long noncoding RNA (lncRNA)
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The potential of Escherichia coli (E. coli) isolated from inflammatory bowel disease (IBD) patients to damage the integrity of the intestinal epithelium was investigated. E. coli strains isolated from patients with ulcerative colitis (UC) and healthy controls were tested for
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