Anti-CKAP5 Antibody

Cytoskeleton-associated protein 5 (CKAP5) is a member of the TOG/XMAP215 family containing heat repeats and plays a role in kinetochore mircotubule protection from depolymerization by MCAK. CKAP5 is the homolog of the Xenopus protein, XMAP25 (also known as chTog), which plays an essential role in centrosomal assembly. CKAP5 is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Studies show CKAP5 interacts with TACC1, TACC3, and tubulin leading to the hypothesis that it functions in spindle microtuble organization. Hepatomas and colonic tumors have shown overexpression of CKAP5.

KLH-conjugated linear peptide corresponding to human CKAP5.

Anti-CKAP5 Antibody is an antibody against CKAP5 for use in IC.

Immunocytochemistry Analysis: A 1:250 dilution from a previous lot detected CKAP5 in RPE1 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CKAP5 in A431 cells.

226 kDa calculated.
Three isoforms are produced by alternative splicing with molecular weights of 225, 218, and 226 kDa

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cells

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