推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse, human
种属反应性(根据同源性预测)
rat (based on 100% sequence homology), canine (based on 100% sequence homology)
技术
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TBC1D4(9882)
mouse ... Tbc1D4(210789)
一般描述
AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.
特异性
Chimpanzee, equine, and ox (90% sequence homology).
This antibody recognizes AS160 at the C-terminus.
免疫原
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of mouse AS160.
应用
Anti-AS160 Antibody detects level of AS160 & has been published & validated for use in WB & IP.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected AS160 on 10 µg of HEPG2 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated AS160 from HEPG2 lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated AS160 from HEPG2 lysate.
质量
Evaluated by Western Blot in HEPG2 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected AS160 on 10 µg of HEPG2 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected AS160 on 10 µg of HEPG2 cell lysate.
目标描述
~ 160 kDa
外形
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HEPG2 cell lysate
HEPG2 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
The journals of gerontology. Series A, Biological sciences and medical sciences, 78(2), 177-185 (2022-10-22)
AMP-activated protein kinase (AMPK), a highly conserved, heterotrimeric serine/threonine kinase with critical sensory and regulatory functions, is proposed to induce antiaging actions of caloric restriction (CR). Although earlier studies assessed CR's effects on AMPK in rodent skeletal muscle, the scope
American journal of physiology. Endocrinology and metabolism, 308(7), E603-E612 (2015-02-12)
Either calorie restriction [CR; consuming 60-65% of ad libitum (AL) intake] or acute exercise can independently improve insulin sensitivity in old age, but their combined effects on muscle insulin signaling and glucose uptake have previously been unknown. Accordingly, we assessed
Lipid mixtures containing a very high proportion of saturated fatty acids only modestly impair insulin signaling in cultured muscle cells.
Testing null
American journal of physiology. Endocrinology and metabolism, 315(5), E859-E871 (2018-08-22)
A single exercise session can increase insulin-stimulated glucose uptake (GU) by skeletal muscle, concomitant with greater Akt substrate of 160 kDa (AS160) phosphorylation on Akt-phosphosites (Thr642 and Ser588) that regulate insulin-stimulated GU. Recent research using mouse skeletal muscle suggested that
The journals of gerontology. Series A, Biological sciences and medical sciences, 71(3), 323-332 (2015-09-06)
Exercise and calorie restriction (CR) can each improve insulin sensitivity in older individuals, but benefits of combining these treatments on skeletal muscle insulin signaling and glucose uptake are poorly understood, especially in predominantly slow-twitch muscles (eg, soleus). Accordingly, our purpose
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