Anti-PKM2 Antibody

Pyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.

This antibody recognizes PKM2 near the C-terminus.

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human PKM2 near the C-terminus.

Anti-PKM2 Antibody is a highly specific rabbit polyclonal antibody, that targets Pyruvate Kinase & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected PKM2 in HeLa cells.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

Evaluated by Western Blotting in P19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PKM2 in 10 µg of P19 cell lysate.

~60 kDa observed

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
P19 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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