Anti-ZnT8

Zinc transporter 8 (UniProt: Q8IWU4; also known as ZnT-8, Solute carrier family 30 member 8) is encoded by the SLC30A8 (also known as ZNT8) gene (Gene ID: 169026) in human. Zinc is required for insulin crystallization, storage, and secretion in the secretory granules of pancreatic β-cells. Delivery of Zn2+ into the insulin-containing granules is largely accomplished by the ZnT8 protein. ZnT8 is a homodimeric, multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and three extracellular domains. It serves as a granule-specific Zn2+ transporter that is abundantly expressed in pancreatic islet cells. It contains a histidine-rich region, HXXXXXHNH-motif, in its third cytoplasmic domain, which serves as a ligand for zinc. ZnT8 is expressed in both a and b cells, but not in  cells. It plays a major role in Zn2+ uptake into the secretory granules. During stimulated secretion, Zn2+ is shown to be co-released with other granular content into the extracellular medium.Loss of ZnT8 function is reported to reduce total islet Zn2+ content as well as free Zn2+ in the cytosol and granules. Loss of ZnT8 also results in reduced dense core granules and increased granule diameter. Studies have shown that the expression of ZnT8 is significantly reduced in the islets of diabetic murine models. Two isoforms of ZnT8 have been described that are produced by alternative splicing. (Ref.: Ghazvini Zadeh, EH., et al. (2020). Cell Reports 32(2); 107904; Dwivedi, OP., et al. (2019). Nat. Genetics. 51(11); 1596-1606).

This rabbit polyclonal antibody detects Zinc transporter 8 (ZnT8). It targets an epitope within the C-terminal, cytoplasmic domain.

GST-tagged recombinant fragment corresponding to 102 amino acids from the C-terminal, cytoplasmic domain of human zinc transporter 9 (ZnT8).

Quality Control Testing

Evaluated by Immunofluorescence in mouse pancreas frozen formalin fixed sections.

Immunofluorescence Analysis: A 1:100 dilution of this antibody detected ZnT8 in mouse pancreas frozen formalin fixed sections.

Tested Applications

Immunofluorescence Analysis: A representative lot detected ZnT8 in Immunofluorescence applications (Zadeh, EHG., et.al. (2020). Cell Rep. 32(2):107904).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ZnT8, Cat. No. ABS2252, is a rabbit polyclonal antibody that detects Zinc transporter 8 (ZnT8) and is tested in Immunofluorescence.

Rabbit polyclonal antibody in 0.05% sodium azide.

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.