Anti-phospho ULK1(Ser746)

Serine/threonine-protein kinase ULK1 (UniProt: O70405; also known as EC:2.7.11.1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1) is encoded by the Ulk1 gene (Gene ID: 22241) in murine species. ULK-1 is a cytosolic serine/threonine protein kinase that is localized in cytosol and preautophagosomal structures. Under condition of starvation, it is localized to punctate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. Its protein kinase domain is localized to amino acids 16-278. It is involved in autophagy in response to starvation and acts upstream of PI3-kinase (PIK3C3) to regulate the formation of autophagophores, the precursors of autophagosomes. It acts both as a downstream effector and a negative regulator of mTORC1 via interaction with Raptor. ULK1 contains multiple phosphorylation sites, and their phosphorylation status is reported to regulate canonical autophagy. Under unstimulated conditions, it is phosphorylated at serine 637 and 757 by mTOR complex that leads to its inactivation. ULK1 is activated via phosphorylation by AMPK and also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. Despite its similarity with ULK2 in its enzymatic domain, there are major differences in their autophagy-related interactors and their post-translational and transcriptional regulators. ULK1 undergoes phosphorylation at serine 746 by receptor interacting protein kinase 3 (RIPK3) during genotoxic stress-induced alternative autophagy and this phosphorylated form is shown to localize exclusively on the Golgi and is essential for alternative autophagy, but not canonical autophagy. (Ref.: Torii, S., et al. (2020). Nat. Commun. 11: 1754).

This rabbit polyclonal antibody detects ULK-1 phosphorylated on serine 746.

KLH-conjugated linear peptide corresponding to 16 amino acids surrounding phosphoserine 746 of mouse ULK-1.

Quality Control Testing

Evaluated by Western blotting with constructs of ULK-1 phosphorylated on Serine 746.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected MBP-tagged ULK1 construct phosphorylated on serine 746, but not in non-phosphorylated construct.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ULK1 phosphorylated on serine 746.

Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho ULK1(Ser746) in Immunoprecipitation applications (Satoru Torii, et al. (2020). Nat. Commun. 11(1):1754).

Immunofluorescence Analysis: A representative lot detected phospho ULK1(Ser746) in Immunofluorescence applications (Satoru Torii, et al. (2020). Nat. Commun. 11(1):1754).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-phospho ULK1(Ser746), Cat. No. ABS2250, is a rabbit polyclonal antibody that detects ULK1 phosphorylated on serine 746 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Purified rabbit polyclonal antibody in buffer containing PBS with 0.09% sodium azide

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.