生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
分子量
calculated mol wt 72.16 kDa
observed mol wt ~54 kDa
种属反应性
rat
种属反应性(根据同源性预测)
mouse, human
包装
antibody small pack of 100 μg
技术
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型
IgG
UniProt登记号
运输
dry ice
储存温度
2-8°C
靶向翻译后修饰
acetylation (Lys105)
基因信息
rat ... Rhot1(303351)
一般描述
Mitochondrial Rho GTPase (UniProt: A1L1L6; also known as Miro1) is encoded by the Rhot1 (also known as Rnf135, rCG34120) gene (Gene ID: 303351) in rat. Miro1 is a single-pass type IV membrane protein with a transmembrane domain (aa 605-627). It functions as a mitochondrial GTPase and is involved in mitochondrial trafficking and is considered to be a potential adaptor for microtubule‐based peroxisome motility in mammalian cells. It plays a key role in mitochondrial motility on microtubules and in homeostasis. In mammals, Miro1 and Miro2 share about 60% sequence homology. Three acetylation sites for Miro1 have been identified in rat that are located on lysines 105, 525, and 629. Acetylation of Miro1 on lysine 105 (K105) is shown to increase mitochondrial transport and axon growth. Exposure of neurons to myelin-associated glycoprotein (MAG) and chondroitin sulfate proteoglycans reduces K105 acetylation of Miro1, which reduces axonal mitochondrial transport. Ac-Miro1 undergoes deacetylation with histone deacetylase 6 (HDAC6) and inhibition of HDAC6 increases Miro1 acetylation in axons. Loss of Miro1 in neurons causes depletion of mitochondria from corticospinal tract axons and leads to progressive neurological deficits similar to upper motor neuron disease. (Ref.: Kalinkski, AL., et al. (2019). J. Cell Biol. 218(6); 1871-1890; Castro, IG., et al. (2018). Traffic. 19(3); 229-242; Nguyen, TT., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(35); E3631-E3640).
特异性
This rabbit polyclonal antibody specifically detects mitochondrial Rho GTPase acetylated on lysine 105. It targets an epitope within 10 amino acids surrounding acetylated lysine 105 from the N-terminal half.
免疫原
Linear peptide corresponding to 10 amino acids surrounding acetylated lysine 105 from the N-terminal half of rat Mitochondrial Rho GTPase (Miro1).
应用
Quality Control Testing
Evaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 μM; 4 h)
Tested Applications
Immunofluorescence Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Immunofluorescence applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
Western Blotting Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Western Blotting applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
ELISA Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in ELISA applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 μM; 4 h)
Tested Applications
Immunofluorescence Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Immunofluorescence applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
Western Blotting Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Western Blotting applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
ELISA Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in ELISA applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Acetyl-Miro1 (Lys 105), Cat. No. ABS2247, is a rabbit polyclonal antibody that detects mitochondrial Rho GTPase (Miro) acetylated on Lysine 105 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
外形
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
储存及稳定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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