Anti-TFAM

Transcription factor A, mitochondrial (UniProt: Q00059; also known as MtTFA, Mitochondrial transcription factor 1, mtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM) is encoded by the TFAM (also known as TCF6, TCF6L2) gene (Gene ID: 7019) in human. TFAM is essential for human mtDNA transcription and acts as a key regulator of mtDNA copy number. It is synthesized as a precursor form with a mitochondrial targeting peptide that is cleaved following import into mitochondria. TFAM consists of an amino-terminal HMG domain, a basic linker region, a second HMG domain and a basic carboxy-terminal tail. TFAM binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. It is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. TFAM is reported to promote transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. It is also able to unwind DNA and bend the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Tissue-specific depletion of TFAM protein is shown to deplete mitochondrial tDNA and causes severe respiratory chain deficiency. (Ref.: Ekstrand, MI et al. (2004). Hum. Mol. Genet. 13(9): 935-944).

This polyclonal antibody specifically detects TFAM in human.

His-Sumo recombinant fusion protein corresponding to 204 amino acids of human TFAM. The signal peptide was removed prior to immunization.

Detect TFAM, mitochondrial using this rabbit polyclonal Anti-TFAM, Cat. No. ABS2083. It has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected TFAM in transfected Hela cell Lysate (Nouws, J., et. al. (2016). J Biol Chem. 291(2):989-97).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TFAM in Huh7.5 cell lysate (Courtesy of Dr. Suresh Sharma, Cameron Laboratory, Pennsylvania State University, University Park, PA).

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFAM in 10 µg of HeLa cell lysate.

~25 kDa observed; 29.00 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.