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生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, monkey
技术
immunofluorescence: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... VPS25(84313)
一般描述
Vacuolar protein-sorting-associated protein 25 (UniProt: Q9BRG1; also known as hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25) is encoded by the VPS25 (also known as DERP9, EAP20) gene (Gene ID: 84313) in human. VPS25 is a component of the ESCRT-II complex (endosomal sorting complex required for transport II) that is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is diffusely distributed throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on co-expression with CHMP6. It is expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression have been reported in heart, skeletal muscle, brain and lung.
特异性
This polyclonal antibody specifically detects Vacuolar protein-sorting-associated protein 25 (VPS25) in human and monkey cells.
免疫原
Epitope: unknown
GST-tagged full length human recombinant VPS25 protein.
应用
Anti-VPS25, Cat. No. ABS1676, is a rabbit polyclonal antibody that targets Vacuolar protein-sorting-associated protein 25 and has been tested for use in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected VPS25 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
Western Blotting Analysis: A 1:500 & 1:1,000 dilution from a representative lot detected VPS25 in lysate of transfected HEK293T cells (Sandrin, V., et. al. (2013). Retrovirology. 10:104; Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
Western Blotting Analysis: A 1:500 & 1:1,000 dilution from a representative lot detected VPS25 in lysate of transfected HEK293T cells (Sandrin, V., et. al. (2013). Retrovirology. 10:104; Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
Research Category
Signaling
Signaling
质量
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected VPS25 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected VPS25 in 10 µg of HEK293 cell lysate.
目标描述
~20 kDa observed; 20.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
法规信息
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