Anti-Exportin-1/CRM1 Antibody

Exportin-1 (UniProt O14980; also known as Chromosome region maintenance 1 protein homolog, CRM1 homolog, Exp1) is encoded by the XPO1 (also known as CRM1) gene (Gene ID 7514) in human. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Most nuclear importins & exportins, including exportin-1, belong to the beta-karyopherin (KAP) family of proteins, Importins bind their cargoes with either nuclear localization signal (NLS) or M9 domain-containing hnRNP proteins in the cytoplasm, while exportins target their substrates via their nuclear export signal (NES) or M9 domain inside the nucleus. The energy dependence of nucleocytoplasmic transport replies on the Ran GTPase system, where the nucleotide exchange factor (NEF) RCC1/Prp20p, the GTPase activating protein (GAP) RanGAP1/Rna1p, and the Ran binding protein (RBP) RanBP1/Yrb1p are arranged asymmetrically to produce a high Ran GTP:Ran GDP ratio in the nucleus and the reverse ratio in the cytoplasm. Exportin 1/CRM1 is reported to bind RanGTP and either Rev or PKI NES peptides in a cooperative manner in vitro and exogenous expression of human CRM1 in Xenopus oocytes is shown to increase the rate of Rev and U snRNA nuclear export.

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of human Exportin-1/CRM1.

Immunocytochemistry Analysis: Representative lots detected Crm1 nuclear localization in murine blastocysts and HeLa-derived HtTA-1 cells (Fornerod, M., et al. (1997). EMBO J. 16(4):807-816), as well as elevated nuclear envelope (NE) Crm1 accumulation in HeLa cells due to nuclear export inhibition upon oxidative stress induction by diethyl maleate (DEM) treatment (Crampton, N., et al. (2009). Mol Biol Cell. 20(24):5106-5116).
Immunoprecipitation Analysis: A representative lot detected enhanced Crm1 association with Nup62, Nup153, and Ran in HeLa cells upon diethyl maleate (DEM) treatment by co-IP (Crampton, N., et al. (2009). Mol Biol Cell. 20(24):5106-5116).
Western Blotting Analysis: A representative lot detected reduced Crm1 level in lysates from HeLa cells upon nuclear export inhibitor leptomycin B (LMB) or oxidant diethyl maleate (DEM) treatment (Crampton, N., et al. (2009). Mol Biol Cell. 20(24):5106-5116).
Electron Microscopy: A representative lot detected Crm1 nuclear localization in HeLa-derived HtTA-1 cells by EM (Fornerod, M., et al. (1997). EMBO J. 16(4):807-816).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Crm1 from COS-7 cell lysates (Boyle, S.M., et al.(1999). J Virol. 73(8):6872-6881).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Exportin-1/CRM1 Antibody is validated for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, Electron Microscopy for the detection of Exportin-1/CRM1.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 of this antibody detected Exportin-1/CRM1 in 10 µg of HeLa cell lysate.

~120 kDa observed. Uncharacterized band(s) may appear in some lysates.

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.